Overview

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  Western blot analysis of 3T3 lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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  Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).