Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Rabbit pAb

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ES8616

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Rabbit pAb
反应性:	Human;Rat;Mouse
别名:	Sodium/potassium-transporting ATPase subunit alpha-1 (Na(+)/K(+) ATPase alpha-1 subunit) (EC 3.6.3.9) (Sodium pump subunit alpha-1)
宿主:	Rabbit
稀释比:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
免疫原:	Synthetic peptide from human protein at AA range: 230-290
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	115kD
基因ID:	476
蛋白序列:	P05023
细胞定位:	Basolateral cell membrane ; Multi-pass membrane protein . Cell membrane, sarcolemma ; Multi-pass membrane protein . Cell projection, axon . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
研究背景:	The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

Western blot analysis of KB Hela lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Na+/K+-ATPase α1 (Phospho-Tyr260) Antibody
Western blot analysis of lysates from 293 cells treated with PMA, using phospho-Na+/K+-ATPase α1 (Phospho-Tyr260) antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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