PRAK rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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PRAK rabbit pAb

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ES7818

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	PRAK rabbit pAb
反应性:	Human;Mouse
别名:	MAPKAPK5; PRAK; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; p38-regulated/activated protein kinase; PRAK
宿主:	Rabbit
稀释比:	WB 1:500-2000;IHC-p 1:50-300
免疫原:	The antiserum was produced against synthesized peptide derived from human MAPKAPK5. AA range:148-197
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	60kD
基因ID:	8550
蛋白序列:	Q8IW41
细胞定位:	Cytoplasm. Nucleus. Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export.
研究背景:	The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012],

Western blot analysis of lysates from MCF-7 cells, using MAPKAPK5 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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