PKC ε rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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PKC ε rabbit pAb

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ES6781

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	PKC ε rabbit pAb
反应性:	Human;Mouse;Rat
别名:	PRKCE; PKCE; Protein kinase C epsilon type; nPKC-epsilon
宿主:	Rabbit
稀释比:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
免疫原:	The antiserum was produced against synthesized peptide derived from human PKC epsilon. AA range:688-737
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	83kD
基因ID:	5581
蛋白序列:	Q02156
细胞定位:	Cytoplasm . Cytoplasm, cytoskeleton . Cell membrane . Cytoplasm, perinuclear region . Nucleus . Translocated to plasma membrane in epithelial cells stimulated by HGF (PubMed:17603037). Associated with the Golgi at the perinuclear site in pre-passage fibroblasts (By similarity). In passaging cells, translocated to the cell periphery (By similarity). Translocated to the nucleus in PMA-treated cells (By similarity). .
研究背景:	protein kinase C epsilon(PRKCE) Homo sapiens Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macro

Western Blot analysis of 453 cells using PKC ε Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from HeLa, Jurkat, and 3T3 cells, treated with PMA 125ng/ml 30', using PKC epsilon Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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