Rho B rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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Rho B rabbit pAb

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ES6040

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	Rho B rabbit pAb
反应性:	Human;Mouse;Rat
别名:	RHOB; ARH6; ARHB; Rho-related GTP-binding protein RhoB; Rho cDNA clone 6; h6
宿主:	Rabbit
稀释比:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
免疫原:	The antiserum was produced against synthesized peptide derived from human RHOB. AA range:99-148
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	22kD
基因ID:	388
蛋白序列:	P62745
细胞定位:	Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Cleavage furrow. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.
研究背景:	function:Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.,miscellaneous:RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects.,PTM:Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.,similarity:Belongs to the small GTPase superfamily. Rho family.,subcellular location:Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.,subunit:Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARGGEF3, RTKN and AKAP13.,

Western blot analysis of RHOB Antibody. The lane on the right is blocked with the RHOB peptide.
Western blot analysis of the lysates from HepG2 cells using RHOB antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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