GR rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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GR rabbit pAb

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ES5666

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	GR rabbit pAb
反应性:	Human;Mouse;Rat
别名:	NR3C1; GRL; Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1
宿主:	Rabbit
稀释比:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
免疫原:	The antiserum was produced against synthesized peptide derived from human GR. AA range:181-230
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	95kD
基因ID:	2908
蛋白序列:	P04150
细胞定位:	[Isoform Alpha]: Cytoplasm . Nucleus . Mitochondrion . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . After ligand activation, translocates from the cytoplasm to the nucleus. In the presence of NR1D1 shows a time-dependent subcellular localization, localizing to the cytoplasm at ZT8 and to the nucleus at ZT20 (By similarity). Lacks this diurnal pattern of localization in the absence of NR1D1, localizing to both nucleus and the cytoplasm at ZT8 and ZT20 (By similarity). .; [Isoform Beta]: Nucleus . Cytoplasm . Expressed predominantly in the nucleus with some expression also detected in the cytoplasm. .; [Isoform Alpha-B]: Nucleus . Cytoplasm . After ligand activation, translocates from the cytoplasm to the nucleus. .
研究背景:	This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking pat

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunofluorescence analysis of A549 cells, using GR Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GR Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HT-29 cells, treated with PMA 125ng/ml 30', using GR Antibody. The lane on the right is blocked with the synthesized peptide.

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