SF3b130 rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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SF3b130 rabbit pAb

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ES5345

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	SF3b130 rabbit pAb
反应性:	Human;Mouse
别名:	SF3B3; KIAA0017; SAP130; Splicing factor 3B subunit 3; Pre-mRNA-splicing factor SF3b 130 kDa subunit; SF3b130; STAF130; Spliceosome-associated protein 130; SAP 130
宿主:	Rabbit
稀释比:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
免疫原:	The antiserum was produced against synthesized peptide derived from human SF3B3. AA range:231-280
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	140kD
基因ID:	23450
蛋白序列:	Q15393
细胞定位:	Nucleus .
研究背景:	This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008],

Western blot analysis of SF3B3 Antibody. The lane on the right is blocked with the SF3B3 peptide.
Western blot analysis of the lysates from HeLa cells using SF3B3 antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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