Overview

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  Immunofluorescence analysis of Hela cell. 1,CYP17A1 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
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  Western Blot analysis of 293 cells using CYP17A1 Polyclonal Antibody diluted at 1:2000
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  Western blot analysis of lysates from Jurkat, A549, and HeLa cells, using Cytochrome P450 17A1 Antibody. The lane on the right is blocked with the synthesized peptide.
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  Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).