DNA pol ι rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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DNA pol ι rabbit pAb

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ES4614

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	DNA pol ι rabbit pAb
反应性:	Human;Rat;Mouse;
别名:	POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B
宿主:	Rabbit
稀释比:	Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
免疫原:	The antiserum was produced against synthesized peptide derived from human POLI. AA range:641-690
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	85kD
基因ID:	11201
蛋白序列:	Q9UNA4
细胞定位:	Nucleus . Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage. .
研究背景:	catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.,similarity:Belongs to the DNA polymerase type-Y family.,similarity:Contains 1 umuC domain.,subcellular location:Accumulates at replication forks after DNA damage.,subunit:Binds REV1L (By similarity). Binds POLH.,tissue specificity:Ubiquitous. Highly expressed in testis.,

Western Blot analysis of HeLa cells using DNA pol ι Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from HeLa cells, using POLI Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HeLa cells using POLI antibody.

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