Overview

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  Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
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  Western Blot analysis of A549, SW620, HT29, Jurkat, mouse kidney, mouse lung cells using CD133 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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  Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30m
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  Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30m