Overview

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  Western Blot analysis of various cells using TRAP100 Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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  Immunofluorescence analysis of HUVEC cells, using MED24 Antibody. The picture on the right is blocked with the synthesized peptide.
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  Western blot analysis of lysates from 293 cells, using MED24 Antibody. The lane on the right is blocked with the synthesized peptide.
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  Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).