Overview

• 
  Western Blot analysis of various cells using Topo IIIβ-1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
• 
  Western blot analysis of lysates from HepG2, COLO205, and HUVEC cells, using TOP3B Antibody. The lane on the right is blocked with the synthesized peptide.
• 
  Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200