NFκB-p100 rabbit pAb-科鹿（武汉）生物科技有限责任公司

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NFκB-p100 rabbit pAb

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ES2943

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

Product name:	NFκB-p100 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	NFKB2; LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human NF-kappaB p100/p52. AA range:833-882
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Molecular Weight:	97kD
GeneID:	4791
Human Swiss-Prot No:	Q00653
Cellular localization:	Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
Background:	nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],

Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using NF-kappaB p100 Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(Immunoway cat:RS23920)was diluted at 1:10000, 37° 1hour.
Immunofluorescence analysis of Hela cell. 1,NFκB-p100 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). ATG5 mouse Monoclonal Antibody(3C7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was dilute
Western Blot analysis of various cells using NFκB-p100 Polyclonal Antibody diluted at 1:1000
Western Blot analysis of K562 cells using NFκB-p100 Polyclonal Antibody diluted at 1:1000

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