APC rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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APC rabbit pAb

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ES1674

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	APC rabbit pAb
反应性:	Human;Mouse;Rat
别名:	APC; DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5
宿主:	Rabbit
稀释比:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
免疫原:	The antiserum was produced against synthesized peptide derived from human APC. AA range:2794-2843
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	310kD
基因ID:	324
蛋白序列:	P25054
细胞定位:	Cell junction, adherens junction . Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Cell projection, ruffle membrane . Cytoplasm . Cell membrane . Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184). Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment (PubMed:19151759). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (PubMed:20937854). .
研究背景:	This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008],

Immunofluorescence analysis of Hela cell. 1,APC Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Galectin-3 Monoclonal Antibody(6G2)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
Western Blot analysis of various cells using APC Polyclonal Antibody diluted at 1:500
Western Blot analysis of COLO205 cells using APC Polyclonal Antibody diluted at 1:500
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using APC Antibody. The picture on the right is blocked with the synthesized peptide.

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