Overview

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  Immunofluorescence analysis of Hela cell. 1,AF-10 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). COX IV Monoclonal Antibody(6C8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
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  Western Blot analysis of various cells using AF-10 Polyclonal Antibody diluted at 1:1000
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  Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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  Western blot analysis of lysates from COLO205 and Jurkat cells, using AF10 Antibody. The lane on the right is blocked with the synthesized peptide.