CREB-1 (phospho Ser142) rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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CREB-1 (phospho Ser142) rabbit pAb

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ES1506

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	CREB-1 (phospho Ser142) rabbit pAb
反应性:	Human;Mouse;Rat
别名:	CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
宿主:	Rabbit
稀释比:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
免疫原:	The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser142. AA range:111-160
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	37kD
基因ID:	1385
蛋白序列:	P16220
细胞定位:	Nucleus .
研究背景:	This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],

Western Blot analysis of various cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1:500
Western Blot analysis of HeLa cells using Phospho-CREB-1 (S142) Polyclonal Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CREB (Phospho-Ser142) Antibody

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