Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
免疫原:
The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser807. AA range:781-830
储存条件:
-20°C/1 year
克隆性:
Polyclonal
抗体亚型:
IgG
浓度:
1 mg/ml
实测条带:
106kD
基因ID:
5925
蛋白序列:
P06400
细胞定位:
Nucleus . During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization. .
研究背景:
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008],