PRK1 (phospho-Thr774)/PRK2 (phospho-Thr816) rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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PRK1 (phospho-Thr774)/PRK2 (phospho-Thr816) rabbit pAb

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ES13956

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	PRK1 (phospho-Thr774)/PRK2 (phospho-Thr816) rabbit pAb
反应性:	Human;Mouse;Rat
别名:	Serine/threonine-protein kinase N1 (EC 2.7.11.13) (Protease-activated kinase 1) (PAK-1) (Protein kinase C-like 1) (Protein kinase C-like PKN) (Protein kinase PKN-alpha) (Protein-kinase C-related kinase 1) (Serine-threonine protein kinase N)
宿主:	Rabbit
稀释比:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
免疫原:	Synthesized phosho peptide around human PRK1 (Thr774) and PRK2 (Thr816)
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	103kD
基因ID:	5585
蛋白序列:	Q16512
细胞定位:	Cytoplasm . Nucleus . Endosome . Cell membrane ; Peripheral membrane protein . Cleavage furrow . Midbody . Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. .
研究背景:	protein kinase N1(PKN1) Homo sapiens The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
Western Blot analysis of 1 NIH/3T3 treated with LPS, 2 NIH/3T3 ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000

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