p70 S6 kinase α (phospho Thr229) rabbit pAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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p70 S6 kinase α (phospho Thr229) rabbit pAb

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ES1390

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

产品名称:	p70 S6 kinase α (phospho Thr229) rabbit pAb
反应性:	Human;Mouse;Rat;Pig
别名:	RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinas
宿主:	Rabbit
稀释比:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
免疫原:	The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr229. AA range:195-244
储存条件:	-20°C/1 year
克隆性:	Polyclonal
抗体亚型:	IgG
浓度:	1 mg/ml
实测条带:	60kD
基因ID:	6198
蛋白序列:	P23443
细胞定位:	Cell junction, synapse, synaptosome . Mitochondrion outer membrane. Mitochondrion. Colocalizes with URI1 at mitochondrion.; [Isoform Alpha I]: Nucleus. Cytoplasm.; [Isoform Alpha II]: Cytoplasm.
研究背景:	ribosomal protein S6 kinase B1(RPS6KB1) Homo sapiens This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013],

Immunofluorescence analysis of Hela cell. 1,p70 S6 kinase α (phospho Thr229) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Caspase-8 Monoclonal Antibody(2G12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
Western Blot analysis of various cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500
Western Blot analysis of LOVO cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500
Immunohistochemistry analysis of paraffin-embedded human brain, using p70 S6 Kinase (Phospho-Thr229) Antibody. The picture on the right is blocked with the phospho peptide.

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