6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
宿主:
Rabbit
稀释比:
Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.
免疫原:
6X His synthetic peptide conjugated to KLH.
储存条件:
-20°C/1 year
克隆性:
Polyclonal
抗体亚型:
IgG
浓度:
1 mg/ml
实测条带:
15kD
研究背景:
The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.