| 产品名称: |
Recombinant Human CD32a (H167,C-6His) |
| 产品描述: |
Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor II-A(H167) is produced by our Mammalian expression system and the target gene encoding Ala36-Ile218 is expressed with a 6His tag at the C-terminus. It is identical to FCGR2A131H/R in the reference. |
| 蛋白编号: |
P12318 |
| 分子量: |
21.1 KDa |
| 表观分子量: |
25-32 KDa, reducing conditions |
| 产品纯度: |
Greater than 95% as determined by reducing SDS-PAGE. |
| 内毒素: |
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| 生物活性: |
Loaded Human CD32a-His(Cat#CS35) on HIS1K Biosensor, can bind Anti-Human HER2 mAb(Cat#GMP-A062) with an affinity constant of 0.6 uM as determined in BLI assay. |
| 复溶: |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 储存条件: |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件: |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
| 研究背景: |
Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating type receptor either has or associates noncovalently with an accessory subunit that has an immunoreceptor tyrosinebased activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. |
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