| 产品名称: |
Recombinant Human EGF |
| 产品描述: |
Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed. |
| 蛋白编号: |
P01133 |
| 分子量: |
6.2 KDa |
| 表观分子量: |
11 KDa, reducing conditions |
| 产品纯度: |
Greater than 95% as determined by reducing SDS-PAGE. |
| 内毒素: |
Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| 生物活性: |
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml. |
| 复溶: |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 储存条件: |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 运输条件: |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below. |
| 研究背景: |
Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites. |
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