VSV-G-Tag Mouse mAb-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

产品中心

VSV-G-Tag Mouse mAb

一键复制产品信息

EM1005

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

下载说明 ①

Overview

产品名称:	VSV-G-Tag Mouse mAb
宿主:	Mouse
稀释比:	WB 1:5,000 IP 1:200 IF 1:1,000
免疫原:	Synthetic Peptide
储存条件:	PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
克隆性:	Monoclonal
抗体亚型:	IgG1
浓度:	1mg/mL
研究背景:	Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation.

IP antibody use：5ug VSV-G Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with VSV-G-tag fusion protein 3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Pr
IF analysis of 293T cells transfected with a VSV-G-tagged protein, using ELKbio VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G)
1ug VSV-G fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000

关闭

在线咨询

Online consultation

在线咨询
技术支持

关注微信公众号

微信扫一扫立即咨询

微信电脑端咨询

微信扫一扫立即咨询

微信电脑端咨询

关闭