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人左旋肉碱/L-肉碱酶联免疫吸附检测试剂盒
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ELK9821
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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产品名称: Human L-Carnitine ELISA Kit
反应性: Human
别名: L-Carnitine
反应类型: Sandwich
灵敏度: 0.119 ng/mL
标准品: 20 ng/mL
检测范围: 0.32-20 ng/mL
样本类型: serum, plasma, urine, tissue homogenates
反应时长: 3.5h
研究领域: Biochemicals
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human L-Carnitine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human L-Carnitine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human L-Carnitine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human L-Carnitine in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
20.00 1.987 1.900
10.00 1.686 1.599
5.00 1.177 1.090
2.50 0.921 0.834
1.25 0.525 0.438
0.63 0.389 0.302
0.32 0.234 0.147
0.00 0.087 0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的L-Carnitine， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 87-95% 91%
EDTA 血浆(n=5) 86-96% 91%
Heparin 血浆(n=5) 79-97% 88%

线性

将添加有L-Carnitine的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 92-103% 81-99% 89-97% 87-98%
EDTA 血浆(n=5) 92-105% 83-92% 85-96% 78-90%
Heparin 血浆(n=5) 92-101% 88-102% 87-102% 85-97%
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