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小鼠抗核抗体(ANA)酶联免疫吸附检测试剂盒
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ELK9820
规格： 价格：
48T ￥1960.00
96T ￥2800.00
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Overview

产品名称: Mouse ANA(anti-nuclear Antibody) ELISA Kit
反应性: Mouse
别名: Anti nuclear Antibody
反应类型: Sandwich
灵敏度: 0.58 ng/mL
标准品: 100 ng/mL
检测范围: 1.57-100 ng/mL
样本类型: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长: 3.5h
研究领域: Biochemical research
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ANA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ANA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ANA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ANA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
100.00 2.035 1.947
50.00 1.602 1.514
25.00 1.251 1.163
12.50 0.828 0.740
6.25 0.555 0.467
3.13 0.321 0.233
1.57 0.232 0.144
0.00 0.088 0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的ANA， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 94-105% 98%
EDTA 血浆(n=5) 83-95% 89%
Heparin 血浆(n=5) 86-99% 92%

线性

将添加有ANA的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 88-103% 81-98% 89-97% 87-98%
EDTA 血浆(n=5) 92-101% 82-98% 92-104% 82-96%
Heparin 血浆(n=5) 88-97% 88-104% 97-108% 83-97%
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