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大鼠磷酸化Akt(S473)酶联免疫吸附检测试剂盒
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ELK9777
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Rat Phospho-Akt (S473) ELISA Kit
反应性: Rat
别名: p-AKT
反应类型: Sandwich
灵敏度: 6.4 pg/mL
标准品: 1000 pg/mL
检测范围: 15.63-1000 pg/mL
样本类型: tissue homogenates, cell lysates and other biological fluids
反应时长: 3.5h
研究领域: Enzyme & Kinase;
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Phospho-Akt. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Phospho-Akt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Phospho-Akt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Phospho-Akt in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
1000.00 2.266 2.172
500.00 1.593 1.499
250.00 1.223 1.129
125.00 0.828 0.734
62.50 0.617 0.523
31.25 0.379 0.285
15.63 0.189 0.095
0.00 0.094 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的Phospho-Akt, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 96-107% 101%
EDTA 血浆(n=5) 83-95% 89%
Heparin 血浆(n=5) 86-99% 92%

线性

将添加有Phospho-Akt的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 91-99% 96-105% 95-103% 95-103%
EDTA 血浆(n=5) 84-98% 93-102% 87-105% 83-92%
Heparin 血浆(n=5) 82-96% 88-101% 90-99% 87-93%
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