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人核因子κB亚基p65(NFκB-p65)酶联免疫吸附检测试剂盒
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ELK9589
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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产品名称: Human NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit
反应性: Human
别名: NFKB3; p65; RelA
反应类型: Sandwich
灵敏度: 0.1 ng/mL
标准品: 10 ng/mL
检测范围: 0.16-10 ng/mL
样本类型: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长: 3.5h
研究领域: Signal transduction;
Uniprot ID: Q04206
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
10.00 1.956 1.858
5.00 1.674 1.576
2.50 1.174 1.076
1.25 0.797 0.699
0.63 0.555 0.457
0.32 0.377 0.279
0.16 0.236 0.138
0.00 0.098 0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的NFκB-p65， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 96-107% 101%
EDTA 血浆(n=5) 79-93% 86%
Heparin 血浆(n=5) 80-97% 88%

线性

将添加有NFκB-p65的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 89-98% 95-103% 99-106% 78-92%
EDTA 血浆(n=5) 82-96% 87-103% 91-97% 81-93%
Heparin 血浆(n=5) 78-91% 87-98% 92-101% 96-105%
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