人磷酸化神经丝蛋白H(pNF-H)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK9302

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产品名称:	Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
反应性:	Human
别名:	TC2; Transcobalamin II
反应类型:	Sandwich
灵敏度:	29 pg/mL
标准品:	5000 pg/mL
检测范围:	78.13-5000 pg/mL
样本类型:	Serum, plasma, tissue homogenates and other biological fluids.
反应时长:	3.5h
研究领域:	Neuro science
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve.