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小鼠核因子κB亚基p65亲和肽(NFκB p65)酶联免疫吸附检测试剂盒
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ELK9154
规格： 价格：
48T ￥1960.00
96T ￥2800.00
96T*5 ￥11900.00
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产品名称: Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit
反应性: Mouse
别名: NF kappa B p65delta3; NFKB3
反应类型: Sandwich
灵敏度: 12.2 pg/mL
标准品: 2000 pg/mL
检测范围: 31.25-2000 pg/mL
样本类型: serum, plasma, tissue homogenates
反应时长: 3.5h
研究领域: Immunology
Uniprot ID: Q04207
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
2000.00 2.054 1.959
1000.00 1.527 1.432
500.00 1.251 1.156
250.00 0.834 0.739
125.00 0.453 0.358
62.50 0.326 0.231
31.25 0.187 0.092
0.00 0.095 0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的NFκB-p65， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 90-107% 98%
EDTA 血浆(n=5) 93-107% 100%
Heparin 血浆(n=5) 85-97% 91%

线性

将添加有NFκB-p65的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 86-94% 81-95% 88-97% 85-92%
EDTA 血浆(n=5) 83-96% 93-102% 86-94% 90-98%
Heparin 血浆(n=5) 92-106% 85-97% 96-105% 88-96%
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