人磷脂酰肌醇甘油生物合成类F蛋白(PIGF)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK9034

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产品名称:	Human PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit
反应性:	Human
别名:	PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1
反应类型:	Competitive Inhibition
灵敏度:	6.2 ng/mL
标准品:	1000 ng/mL
检测范围:	15.6-1000 ng/mL
样本类型:	Serum, plasma, other biological fluids
反应时长:	2h
研究领域:	Signal transduction;
Uniprot ID:	Q07326
检测原理:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIGF in the samples is then determined by comparing the OD of the samples to the standard curve.