鸡天冬氨酸转氨酶(AST)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK8734

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产品名称:	Chicken AST(Aspartate Aminotransferase) ELISA Kit
反应性:	Chicken
别名:	cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble
反应类型:	Sandwich
灵敏度:	0.116 U/L
标准品:	20 U/L
检测范围:	0.32-20 U/L
样本类型:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Enzyme & Kinase;Tumor immunity;Infection immunity;Hepatology;
Uniprot ID:	P00504
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken AST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken AST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken AST in the samples is then determined by comparing the OD of the samples to the standard curve.