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人黑色素(Melanin)酶联免疫吸附检测试剂盒
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ELK8367
规格： 价格：
48T ￥1680.00
96T ￥2400.00
96T*5 ￥10200.00
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产品名称: Human Melanin ELISA Kit
反应性: Human
别名: melanin
反应类型: Sandwich
灵敏度: 12.9 pg/mL
标准品: 1000 pg/mL
检测范围: 15.63-1000 pg/mL
样本类型: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长: 3.5h
研究领域: Cytokine;Infection immunity;
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human melanin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human melanin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human melanin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human melanin in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
1000.00 1.948 1.844
500.00 1.547 1.443
250.00 1.223 1.119
125.00 0.872 0.768
62.50 0.516 0.412
31.25 0.357 0.253
15.63 0.202 0.098
0.00 0.104 0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的melanin， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 80-94% 87%
EDTA 血浆(n=5) 91-105% 98%
Heparin 血浆(n=5) 85-97% 91%

线性

将添加有melanin的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 83-101% 90-99% 87-103% 89-97%
EDTA 血浆(n=5) 87-97% 86-92% 89-103% 89-98%
Heparin 血浆(n=5) 79-85% 87-96% 82-95% 89-103%
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