产品中心

色氨酸(Trp)酶联免疫吸附检测试剂盒
一键复制产品信息
ELK8262
规格： 价格：
48T ￥1960.00
96T ￥2800.00
96T*5 ￥11900.00
下载说明 ①

Overview 文献(2)

产品名称: Trp(Tryptophan) ELISA Kit
反应性: General
别名: Trp
反应类型: Competitive Inhibition
灵敏度: 0.6 µg/mL
标准品: 100 µg/mL
检测范围: 1.57-100 µg/mL
样本类型: serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Enzyme & Kinase;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Trp. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Trp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Trp in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (µg/mL) OD Corrected OD
100.00 0.183
50.00 0.389
25.00 0.567
12.50 0.895
6.25 1.156
3.13 1.537
1.57 1.762
0.00 2.115

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板件的精密度。

回收率

分别往不同样本中添加已知浓度的Trp， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 89-103% 96%
EDTA 血浆(n=5) 88-102% 95%
Heparin 血浆(n=5) 92-105% 99%

线性

将添加有Trp的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-96% 86-98% 91-103% 87-93%
EDTA 血浆(n=5) 85-94% 92-101% 87-96% 82-90%
Heparin 血浆(n=5) 87-101% 97-106% 86-97% 92-99%
关闭

在线咨询

Online consultation

  • 在线咨询
  • 技术支持

关注微信公众号

微信扫一扫立即咨询

微信电脑端咨询

微信扫一扫立即咨询

微信电脑端咨询
关闭