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花生四烯酸(AA)酶联免疫吸附检测试剂盒
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ELK7841
规格： 价格：
48T ￥1960.00
96T ￥2800.00
96T*5 ￥11900.00
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产品名称: AA(Arachidonic Acid) ELISA Kit
反应性: General
别名: ARA
反应类型: Competitive Inhibition
灵敏度: 0.93 µg/mL
标准品: 200 µg/mL
检测范围: 3.13-200 µg/mL
样本类型: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Signal transduction;Metabolic pathway;Endocrinology;Neuro science;Hormone metabolism;Bone metabolism;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (µg/mL) OD Corrected OD
200.00 0.235
100.00 0.336
50.00 0.524
25.00 0.897
12.50 1.275
6.25 1.572
3.13 1.755
0.00 2.325

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的AA， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 78-92% 85%
EDTA 血浆(n=5) 88-106% 97%
Heparin 血浆(n=5) 81-95% 90%

线性

将添加有AA的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 91-99% 88-97% 96-105% 95-103%
EDTA 血浆(n=5) 85-105% 87-98% 91-102% 86-102%
Heparin 血浆(n=5) 82-96% 85-97% 85-94% 87-96%
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