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叶酸(FA)酶联免疫吸附检测试剂盒
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ELK7834
规格： 价格：
48T ￥1680.00
96T ￥2400.00
96T*5 ￥10200.00
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Overview 文献(1)

产品名称: FA(Folic Acid) ELISA Kit
反应性: General
别名: VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate
反应类型: Competitive Inhibition
灵敏度: 14 pg/mL
标准品: 10000 pg/mL
检测范围: 156.25-10000 pg/mL
样本类型: serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Hematology;Reproductive science;Nutrition metabolism;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with FA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
10000.00 0.212
5000.00 0.397
2500.00 0.583
1250.00 0.857
625.00 1.152
312.50 1.511
156.25 1.715
0.00 2.226

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的FA， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 82-95% 88%
EDTA 血浆(n=5) 80-94% 87%
Heparin 血浆(n=5) 89-101% 88%

线性

将添加有FA的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 80-101% 87-96% 86-97% 88-95%
EDTA 血浆(n=5) 85-94% 87-96% 92-101% 82-98%
Heparin 血浆(n=5) 85-94% 85-94% 79-96% 85-99%
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