小鼠磷酸肌醇-3-激酶3(PIK3C3)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK7518

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产品名称:	Mouse PIK3C3(Phosphoinositide-3-Kinase Class 3) ELISA Kit
反应性:	Mouse
别名:	Vps34; Phosphatidylinositol 3-kinase p100 subunit; Phosphatidylinositol 3-kinase catalytic subunit type 3
反应类型:	Sandwich
灵敏度:	0.28 ng/mL
标准品:	50 ng/mL
检测范围:	0.79-50 ng/mL
样本类型:	tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Enzyme & Kinase;
Uniprot ID:	Q6PF93
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIK3C3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIK3C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIK3C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIK3C3 in the samples is then determined by comparing the OD of the samples to the standard curve.