大鼠O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK7160

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产品名称:	Rat MGMT(O-6-Methylguanine DNA Methyltransferase) ELISA Kit
反应性:	Rat
别名:	Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase
反应类型:	Sandwich
灵敏度:	27 pg/mL
标准品:	5000 pg/mL
检测范围:	78.13-5000 pg/mL
样本类型:	Tissue homogenates and other biological fluids.
反应时长:	3.5h
研究领域:	Signal transduction;Enzyme & Kinase;Tumor immunity;
Uniprot ID:	P24528
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MGMT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MGMT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MGMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MGMT in the samples is then determined by comparing the OD of the samples to the standard curve.