大鼠线粒体解偶联蛋白1(UCP1)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK6326MS

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产品名称:	Rat UCP1(Uncoupling Protein 1, Mitochondrial) Microsample ELISA Kit
反应性:	Rat
别名:	SLC25A7; UCP; Thermogenin; Solute carrier family 25 member 7
反应类型:	Sandwich
灵敏度:	0.113 ng/mL
标准品:	20 ng/mL
检测范围:	0.32-20 ng/mL
样本类型:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长:	3.5h
研究领域:	Signal transduction;
Uniprot ID:	P04633
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat UCP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat UCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat UCP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat UCP1 in the samples is then determined by comparing the OD of the samples to the standard curve.