小鼠心肌肌钙蛋白T(cTnT/TNNT2)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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小鼠心肌肌钙蛋白T(cTnT/TNNT2)酶联免疫吸附检测试剂盒

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ELK6207

规格：	价格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

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Overview 文献(6)

Product name:	Mouse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac
Assay Type:	Sandwich
Sensitivity:	5.5 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular biology;
Uniprot ID:	P50752
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Frontiers in Cellular and Infection Microbiology

New insights of necroptosis and immune infiltration in sepsis-induced myocardial dysfunction from bioinformatics analysis through RNA-seq in mice

日期：2022-12-15 IF：6.073
Molecular Medicine

Metrnl ameliorates myocardial ischemia–reperfusion injury by activating AMPK-mediated M2 macrophage polarization

日期：2025-04-17 IF：6
BMC Infectious Diseases

Integrated bioinformatics and experiment validation reveal cuproptosis-related biomarkers and therapeutic targets in sepsis-induced myocardial dysfunction

日期：2025-04-17 IF：3.4
Infection and Drug Resistance

Validation of Senescence of the Role of ATM/P53 Pathway in Myocardial Senescence in Mice with Sepsis

日期：2025-05-13 IF：2.9
Pharmacognosy Journal

Analysis Protein APOB and TroponinT in Obese Mice (Musmusculus) Induced by Static Magnetic Field as a Marker of Coronary Heart

日期：2022-10-30 IF：-
Pharmacognosy Journal

Analysis Protein APOB and TroponinT in Obese Mice (Musmusculus) Induced by Static Magnetic Field as a Marker of Coronary Heart

日期：2022-08-13 IF：0

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.952	1.856
500.00	1.586	1.490
250.00	1.147	1.051
125.00	0.895	0.799
62.50	0.547	0.451
31.25	0.354	0.258
15.63	0.142	0.046
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant cTnT/TNNT2 and the recovery rates were calculated by comparing the measured value to the expected amount of cTnT/TNNT2 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	78-92%	85%
Heparin plasma(n=5)	78-96%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cTnT/TNNT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.