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小鼠神经肽FF(NPFF)酶联免疫吸附检测试剂盒
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ELK6090
规格: 价格:
48T ¥1960.00
96T ¥2800.00
96T*5 ¥11900.00

Overview

产品名称: Mouse NPFF(Neuropeptide FF) ELISA Kit
反应性: Mouse
别名: FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF
反应类型: Competitive Inhibition
灵敏度: 3.69 pg/mL
标准品: 500 pg/mL
检测范围: 7.82-500 pg/mL
样本类型: serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Neuro science;
Uniprot ID: Q9WVA8
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NPFF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NPFF in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
500.00 0.189
250.00 0.386
125.00 0.575
62.50 0.823
31.25 1.157
15.63 1.592
7.82 1.793
0.00 2.211

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的NPFF, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 87-93% 90%
EDTA 血浆(n=5) 80-90% 85%
Heparin 血浆(n=5) 84-106% 92%

线性

将添加有NPFF的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-92% 90-96% 87-98% 79-92%
EDTA 血浆(n=5) 95-103% 91-105% 78-90% 89-97%
Heparin 血浆(n=5) 79-95% 93-106% 85-95% 92-104%
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