大鼠VGF神经生长因子诱导蛋白(VGF)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK6057MS

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产品名称:	Rat VGF(VGF Nerve Growth Factor Inducible) Microsample ELISA Kit
反应性:	Rat
别名:	NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide
反应类型:	Sandwich
灵敏度:	19.72 pg/mL
标准品:	3000 pg/mL
检测范围:	46.88-3000 pg/mL
样本类型:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长:	3.5h
研究领域:	Metabolic pathway;Infection immunity;Neuro science;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VGF in the samples is then determined by comparing the OD of the samples to the standard curve.