大鼠白介素7受体(IL7R)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

一键复制产品信息

ELK5749MS

下载说明 ①

产品名称:	Rat IL7R(Interleukin 7 Receptor) Microsample ELISA Kit
反应性:	Rat
别名:	CDW127; CD127; IL-7R-Alpha
反应类型:	Sandwich
灵敏度:	0.122 ng/mL
标准品:	20 ng/mL
检测范围:	0.32-20 ng/mL
样本类型:	Serum, plasma and other biological fluids.
反应时长:	3.5h
研究领域:	Signal transduction;CD & Adhesion molecule;Infection immunity;Immunodeficiency;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL7R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL7R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL7R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL7R in the samples is then determined by comparing the OD of the samples to the standard curve.