大鼠蛋白酶3(PR3)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK5641MS

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产品名称:	Rat PR3(Proteinase 3) Microsample ELISA Kit
反应性:	Rat
别名:	MBN; PRTN3; ACPA; AGP7; C-ANCA; MBT; P29; PRK; Myeloblastin; Serine Proteinase,Neutrophil,Wegener Granulomatosis Autoantigen; Protease K; Proteinase K; Endopeptidase K
反应类型:	Sandwich
灵敏度:	1.34 ng/mL
标准品:	200 ng/mL
检测范围:	3.13-200 ng/mL
样本类型:	Serum, plasma and other biological fluids.
反应时长:	3.5h
研究领域:	Enzyme & Kinase;Infection immunity;Autoimmunity;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PR3 in the samples is then determined by comparing the OD of the samples to the standard curve.