人家族性地中海热基因(MEFV)微量上样酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人家族性地中海热基因(MEFV)微量上样酶联免疫吸附检测试剂盒

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ELK5506MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

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Overview

Product name:	Human MEFV(Mediterranean Fever) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	MEF; FMF; TRIM20; Pyrin; Marenostrin
Assay Type:	Sandwich
Sensitivity:	12.6 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MEFV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEFV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MEFV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEFV in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.059	1.968
1000.00	1.504	1.413
500.00	1.263	1.172
250.00	0.897	0.806
125.00	0.615	0.524
62.50	0.317	0.226
31.25	0.216	0.125
0.00	0.091	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MEFV and the recovery rates were calculated by comparing the measured value to the expected amount of MEFV in samples.

Matrix	Recovery range	Average
serum(n=5)	84-104%	94%
EDTA plasma(n=5)	89-99%	94%
Heparin plasma(n=5)	87-105%	96%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MEFV and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.