人Ⅲ型前胶原羧基端原肽(PIIICP)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK5493

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产品名称:	Human PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit
反应性:	Human
别名:	P3CP; C-Propeptide Of Type III Procollagen; Procollagen III Carboxy Terminal Propeptide
反应类型:	Competitive Inhibition
灵敏度:	0.96 ng/mL
标准品:	200 ng/mL
检测范围:	3.13-200 ng/mL
样本类型:	serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长:	2h
研究领域:	Metabolic pathway;Bone metabolism;
检测原理:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIIICP in the samples is then determined by comparing the OD of the samples to the standard curve.