人糖化血红蛋白A1c(HbA1c)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK5487MS

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产品名称:	Human HbA1c(Glycated Hemoglobin A1c) Microsample ELISA Kit
反应性:	Human
别名:	Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
反应类型:	Sandwich
灵敏度:	2.23 ng/mL
标准品:	500 ng/mL
检测范围:	7.81-500 ng/mL
样本类型:	plasma
反应时长:	3.5h
研究领域:	Diagnosis of diabetes mellitus
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HbA1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.