人核受体亚家族1D组成员1(NR1D1)微量上样酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人核受体亚家族1D组成员1(NR1D1)微量上样酶联免疫吸附检测试剂盒

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ELK5412MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

Product name:	Human NR1D1(Nuclear Receptor Subfamily 1, Group D, Member 1) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	THRA1; EAR1; THRAL; ear-1; hRev; Rev-ErbA Alpha; V-erbA-related protein 1
Assay Type:	Sandwich
Sensitivity:	12.1 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;
Uniprot ID:	P20393
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NR1D1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NR1D1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NR1D1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NR1D1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	1.992	1.909
1000.00	1.573	1.490
500.00	1.103	1.020
250.00	0.858	0.775
125.00	0.524	0.441
62.50	0.350	0.267
31.25	0.174	0.091
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NR1D1 and the recovery rates were calculated by comparing the measured value to the expected amount of NR1D1 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-104%	97%
EDTA plasma(n=5)	90-130%	110%
Heparin plasma(n=5)	89-101%	95%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NR1D1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.