人过氧化物酶体生物合成因子2(PEX2)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK5278MS

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产品名称:	Human PEX2(Peroxisomal Biogenesis Factor 2) Microsample ELISA Kit
反应性:	Human
别名:	PXMP3; PAF1; PMP35; PMP3; RNF72; Peroxin 2; Peroxisome Assembly Factor 1; Peroxisomal Membrane Protein 35kDa; Peroxisomal Membrane Protein 3
反应类型:	Sandwich
灵敏度:	0.061 ng/mL
标准品:	10 ng/mL
检测范围:	0.16-10 ng/mL
样本类型:	Tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Developmental science;
Uniprot ID:	P28328
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PEX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PEX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PEX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PEX2 in the samples is then determined by comparing the OD of the samples to the standard curve.