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小鼠转化生长因子β2(TGFb2)酶联免疫吸附检测试剂盒
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ELK5216
规格: 价格:
48T ¥1680.00
96T ¥2400.00
96T*5 ¥10200.00

Overview

产品名称: Mouse TGFb2(Transforming Growth Factor Beta 2) ELISA Kit
反应性: Mouse
别名: TGF-B2; TGF-Beta2; G-TSF;  LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor
反应类型: Competitive Inhibition
灵敏度: 8.95 pg/mL
标准品: 2000 pg/mL
检测范围: 31.25-2000 pg/mL
样本类型: Serum, plasma, cell culture supernates and other biological fluids
反应时长: 2h
研究领域: Cytokine;Infection immunity;
Uniprot ID: P27090
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TGFb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
2000.00 0.197
1000.00 0.385
500.00 0.611
250.00 0.812
125.00 1.157
62.50 1.583
31.25 1.796
0.00 2.213

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的TGFb2, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 80-96% 88%
EDTA 血浆(n=5) 82-98% 90%
Heparin 血浆(n=5) 84-98% 96%

线性

将添加有TGFb2的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 82-93% 96-105% 83-95% 88-103%
EDTA 血浆(n=5) 95-103% 88-96% 81-95% 91-102%
Heparin 血浆(n=5) 86-93% 85-98% 89-102% 85-98%
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