人CD3d分子(CD3d)微量上样酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK5191MS

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产品名称:	Human CD3d(Cluster Of Differentiation 3d) Microsample ELISA Kit
反应性:	Human
别名:	CD3-DELTA; T3D; T-Dell Surface Glycoprotein CD3 Delta Chain; T-cell receptor T3 delta chain
反应类型:	Sandwich
灵敏度:	0.112 ng/mL
标准品:	20 ng/mL
检测范围:	0.32-20 ng/mL
Sample type:	Serum, plasma and other biological fluids.
Assay length:	3.5h
研究领域:	Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Uniprot ID:	P04234
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD3d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD3d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD3d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD3d in the samples is then determined by comparing the OD of the samples to the standard curve.